Microfabricated glass devices for rapid single cell immobilization in mouse zygote microinjection.
نویسندگان
چکیده
This paper presents the design and microfabrication of a vacuum-based cell holding device for single-cell immobilization and the use of the device in mouse zygote microinjection. The device contains many through-holes, constructed via two-sided glass wet etching and polydimethylsiloxane (PDMS)-glass bonding. Experimental results of mouse zygote immobilization and microinjection demonstrate that the device is effective for rapid cell immobilization and does not produce negative effect on embryonic development.
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ورودعنوان ژورنال:
- Biomedical microdevices
دوره 11 6 شماره
صفحات -
تاریخ انتشار 2009